INVITRO REGENERATION VIA MULTIPLE SHOOT INDUCTION FROM IMMATURE EMBRYOS OF PIGEONPEA (Cajanus cajan)
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چکیده
Pigeonpea is an important grain legume, however the genetic transformation of pigeonpea has been very challenging to date. In the present study, we describe a simple protocol for plantlet regeneration via multiple shoot induction from 12-16 day old immature embryos of pigeonpea cultivar ICPL 87 which can be used for efficient transformation. By optimizing the culture medium, we have achieved organogenesis via multiple shoot induction on Murashige & Skoog’s medium (MS) medium containing 2, 3 and 4mg/l benzylaminopurine (BAP) from 12-16 day old immature embryos. MS medium fortified with 0.1mg/l NAA and 0.3mg/l GA3 was optimum for shoot elongation, while half concentration MS medium fortified with 0.5mg/l naphthalene acetic acid (NAA) favoured rooting.
منابع مشابه
24 Pages Fermo_6.cdr
A method for regenerating pigeonpea {Cajanus cajan (L.) Millsp.} plants has been developed using cotyledonary nods and embroyonic axes of mature seeds as explants. A large number of shoot buds were induced directly from explants of genotypes Pusa-9 and Bahar when cultured on different basal media fortified with a range of BAP concentrations (0–2 mg/l) and 0–0.5 mg/l gibberellic acid (GA ) eithe...
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Pigeonpea (Cajanus cajan (L.) Millspaugh), a diploid (2n = 22) legume crop with a genome size of 852 Mbp, serves as an important source of human dietary protein especially in South East Asian and African regions. In this study, the draft chloroplast genomes of Cajanus cajan and Cajanus scarabaeoides (L.) Thouars were generated. Cajanus scarabaeoides is an important species of the Cajanus gene p...
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تاریخ انتشار 2013